International Journal of Environmental Sciences

Volume 5 Issue 2 2014- September 2014    Pages: 353-371 <<Previous    Next>>

Studies on Alkaline-thermostable protease from an alkalophilic bacterium: Production, characterization and applications

Author Information:

Aditya Bharadwaj1, Neena Puri2, Prakram Singh Chauhan1, Balneek Singh Cheema1, Naveen Gupta1
1- Department of Microbiology, South Campus Panjab University, Sec-25, Chandigarh, India.
2- Department of Industrial Microbiology, Guru Nanak Khalsa College, Yamunanagar, Haryana, India.


Alkalophilic organisms producing alkaline thermostable protease(s) were isolated from the industry waste, corpses and soil. Out of them Isolate no. NB-34 was found to produce maximum alkaline thermostable protease. Optimization studies on protease production from isolate NB-34 revealed that protease production was maximum : With 0.5% gelatin as nitrogen source, pH 11.2 (2% sodium carbonate, 0.5% glucose, 24 hours incubation period, 37⁰C incubation temperature, 150 rpm agitation. The alkaline thermostable protease from isolate NB-34 had pH and temperature optima of 9.5 and 50⁰C respectively. The Km value for the enzyme was calculated to be 0.909 mg casein/ml and Vmax 5.55. The alkaline thermostable protease from isolate NB-34 was found to be compatible with most of the commercial detergents. When the alkaline thermos table protease from isolate NB-34 was supplemented with the commercial detergents, it improved the destaining capacity of the detergent.

Keywords: Optimization, thermostable protease, alkaline stable mannanase, detergent industry


© 2014 Copyright by the authors, licensee Integrated Publishing Association.This is an open access article distributed under the Creative Commons Attribution License (3.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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