International Journal of Environmental Sciences

Volume 5 Issue 3 2014- November 2014    Pages: 623- 633  <<Previous    Next>>

Chlamydomonas reinhardtii modulates and decreases cytotoxic and genotoxic effects of CUSO4

Author Information:

Svetla Gateva, Gabriele Jovtchev, Zhana Mitrovska, Mila Stergios, Stephka Chankova- Division of Environmental Mutagenesis and Genetic Risk Assessments, Department of Ecosystem Research, Environmental Risk Assessment and Conservation Biology, Institute of Biodiversity and Ecosystem Research, BAS, 2 Gagarin Street, 1113 Sofia, Bulgaria.

ABSTRACT

One of the promising waste removal methods involves the treatment of contaminants with microalgae. Questions we addressed in this work are: i) whether Chlamydomonas reinhardtii possesses ability to modulate the cytotoxic and genotoxic potential of CuSO4; ii) whether the products released in the nutrition medium as a result of algal normal living activity possess cytotoxic and genotoxic activities and could modulate the harmful effect of CuSO4. Algal supernatants and cell extracts are applied in different experimental designs. Two test-systems at different levels of hierarchical scale were used - a higher plant and human lymphocytes, because it is well known that heavy metals are presented in different trophic levels. Mitotic index was used as endpoint for cytotoxicity, the frequency of chromosome aberrations - as endpoint for genotoxicity. Our finding demonstrates that: i) Chlamydomonas reinhardtii and its metabolic products can modulate the harmful effect of CuSO4; ii) this effect is expressed as a reduction, but not as complete elimination of the cytotoxic and genotoxic effects of copper. Our study provides additional experimental evidence that Chlamydomonas reinhardtii can be used for treatment of contaminants such as copper in order to decrease its harmful effect and thereby leading to reduction of health risks.

Keywords: Copper sulphate, Chlamydomonas reinhardtii, Hordeum vulgare, human lymphocytes, genotoxicity..

.DOI:10.6088/ijes.2014050100056

© 2014 Copyright by the authors, licensee Integrated Publishing Association.This is an open access article distributed under the Creative Commons Attribution License (3.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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