International Journal of Environmental Sciences

Volume 6 Issue 3 2015- July 2015  Pages: 338- 344 <<Previous    Next>>

Studies on the sensitivity and biochemical response of fish, Oreochromis Mossambicus to Neem 

Author Information:

Durairaj S, Ramadass N, Arumugam S - Department of zoology, Arignar Anna Government Arts College,Cheyyar- 604 407, Tamil Nadu

ABSTRACT

Aquatic inhabitants have been affected by a number of anthropogenic agents which pollute the aquatic environment. Fish, one of the aquatic inhabitants, is considered as bio indicator of aquatic pollution. The utilization and over usage of synthetic organic chemicals in the form of pesticides contaminate the aquatic environment. In recent past, plant derived products are being widely used as an alternative to pesticides for the pest management and such chemicals also affect the fish in the water bodies. In the present investigation, the plant derived compound neem is used to find out its sensitivity towards the fish to understand the threshold level to minimize the contamination of plant derived commercial compounds in order to avoid the consequences of such contamination. The widely used commercial neem product is used to determine the LC50 level to the fish, Oreochromis mossambicus and it was 0.4ml/l. The regression equation was also derived as Y= -12.323+6.626X. While determining the LC50 value, swimming impairment and increase of opercular activity have been observed.  Besides, to understand the physiological status of O. mossambicus in the various concentrations of neem, protein was analyzed at the end of 96 hrs in the tissues such as gills, liver, muscle and brain since the protein is considered as a biochemical marker. In the various concentrations of neem at 96 hrs, protein level was significantly (P<0.05) reduced in all the tissues. The drastic level of protein decrease indicates the sensitivity of the fish towards the neem.

Keywords: Fish Oreochromis mossambicus, neem, LC50 determination, protein analysis,  tissues of fish.

DOI:10.6088/ijes.6038

© 2015 Copyright by the authors, licensee Integrated Publishing Association.This is an open access article distributed under the Creative Commons Attribution License (3.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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