International Journal of Environmental Sciences

Volume 6 Issue 6 2016- May 2016 Pages: 1113 - 1116 <<Previous    Next>>

Cypermethrin induced DNA damage in Labio Rohita assessed by comet assay

Author Information:

Mohini Gadhia1, Rakesh Prajapati2 and Pankaj Gadhia3
1- Department of Aquatic Biology, Veer Narmad South Gujarat University, Saurat.

2- Department of Zoology, H & HB Kotak Institute of Science, Rajkot

3- S. N. Gene Laboratory and Research Centre, President Plaza-A Ring Road, Surat.

ABSTRACT

Toxicants exposure on fish may induce genetic alterations that can be used as genotoxic markers. We evaluated DNA damage using a Single Cell Gel Electrophoresis assay (SCGE), applied on erythrocytes after exposure of Labeo rohita to sub-lethal (0.03 ppm), lethal (0.06 ppm) and acute lethal (0.10 ppm) concentrations of Cypermethrin.  Results revealed a significant level of DNA damages at all concentrations tested. Damage index of comet assay in L. rohita’s erythrocytes showed significant differences between sublethal, lethal and acute lethal exposures.

Keywords: Comet assay, Labeo rohita, DNA damage, Cypermethrin. Erythrocytes

DOI:10.6088/ijes.6105

© 2016 Copyright by the authors, licensee Integrated Publishing Association.This is an open access article distributed under the Creative Commons Attribution License (3.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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