International Journal of Environmental Sciences

Volume 3 Issue 5 2013- March 2013    Pages: 1314-1321  <<Previous    Next>>

Isolation and identification of petroleum hydrocarbon degrading microorganisms from oil contaminated environment

Author Information:

Panda S. K, Kar R. N, Panda C. R

Institute of Minerals and Materials Technology, Bhubaneswar, Odisha, India

ABSTRACT

Oil contaminated soil, sea water and sediment samples were collected from old oil jetty of Paradip port, Orissa. These samples were screened for bacterial oil degradation using 0.5% diesel in Bushnell-Hass Mineral Salt medium. Samples were incubated separately in shaking orbital incubator at 370C at 125rpm up to 15days. The level of petroleum hydrocarbon degradation was determined by gravimetric assay at each 5 days interval. One potent Gram negative bacillus bacteria was isolated on the basis of petroleum hydrocarbon degradation efficiency and identified as Pseudomonas sp. The bacterial strain had shown 49.93% of diesel oil degradation in 20days against 0.5% of diesel in 100ml BHMS.

Keywords: Biodegradation, Bacteria, Diesel oil, Marine environment

DOI:10.6088/ijes.2013030500001

© 2013 Copyright by the authors, licensee Integrated Publishing Association.This is an open access article distributed under the Creative Commons Attribution License (3.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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