International Journal of Environmental Sciences

Volume 3 Issue 6 2013- June 2013    Pages: 2304-22312  <<Previous    Next>>

Isolation and characterization of microorganisms from mangrove soil of CBD Belapur creek , Navi Mumbai ,MS India

Author Information:

Bhat M.R, Shewade Leena
Department of Biotechnology and Bioinformatics, Pd. Dr. D. Y. Patil University, Plot no. 50, Sector-15,CBD Belapur, Navi Mumbai,Kokan Bhavan 400614, MS, India


In the present study, mangrove soil samples from five different stations of CBD Belapur (19° 1′ 25.33″ N, 73° 2′ 27.65″ E) Navi-Mumbai, MS, India, were collected for the isolation of micro-organisms. Among the soil samples screened, all gram positive isolates were obtained. Out of all the isolates, fourteen distinct isolates were selected and characterized. On the basis of biochemical tests, the isolates were assigned to the genera Bacillus.All the isolates demonstrated very strong L-asparginase activity, whereas few were positive for Protease and Amylase activity. Isolates were observed to grow at pH ranging from 7-8 and had temperature optima of 280C and few had 550C. Most of the organisms could tolerate about 300ppm of heavy metals in the form of AlCl3, MnSO4 and ZnSO4; upto 1000ppm of K2CrO4 and only about 10 ppm of HgCl2 . In addition, a few of these isolates could tolerate more than 8% NaCl. Antibiotic efficacy of the isolates was studied, in which most of the organisms were sensitive to antibiotics tested except Penicillin G.

Keywords: Asparaginase, biochemical characterization, mangrove, heavy metal, saline ecology.


© 2013 Copyright by the authors, licensee Integrated Publishing Association.This is an open access article distributed under the Creative Commons Attribution License (3.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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