International Journal of Environmental Sciences

Volume 2 Issue 3 2012           Pages: 1135- 1143

Detection of novel probiotic bacterium Lactobacillus spp. in the workers of Indian honeybee, Apis cerana indica

Author Information:

Mahesh Pattabhiramaiah- Centre for Apiculture studies, Dept. of Zoology, Bangalore University, Jnana Bharathi, Bangalore-560056, India

Reddy.M.S - Centre for Apiculture studies, Dept. of Zoology, Bangalore University, Jnana Bharathi, Bangalore-560056, India

Dorothea Brueckner - Honeybee Research Unit, University of Bremen, FB 2, 28334 Bremen

ABSTRACT
Many insects are known to have microorganisms in the gut which can play an important role in their nutrition. In the present study, we report the presence of probiotic bacterium Lactobacillus spp. in the gut of the honeybee sub species Apis cerana indica collected from different parts of Karnataka, India which play a very significant role in the general health maintenance of the host. Total bacterial genomic DNA was extracted from the midguts of the worker honeybee sub species Apis cerana indica, collected from different parts of Karnataka and amplified using PCR, with 16S rRNA primers. The amplified PCR products were purified and sequenced directly. This partial, 16S rDNA sequences from Apis cerana indica revealed the presence of novel bacterial flora composed of lactic acid bacteria (LAB), which originated in the honey stomach of the Indian honeybee (Genbank accession number: EU392167) which has a putative health-conferring properties of probiotics.

Keywords : Apis cerana indica, LAB, PCR, Genbank, Karnataka

DOI: 10.6088/ijes.00202030002


© 2012 Copyright Mahesh Pattabhiramaiah et al, licensee IPA.This is an open access article distributed under the Creative Commons Attribution License (2.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The electronic version of the article can be downloaded below.

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