International Journal of Environmental Sciences

Volume 2 Issue 3 2012           Pages:1605- 1608

Antibacterial analysis of crude extracts from the leaves of Tagetes erecta and Cannabis sativa

Author Information:

Das B- Institute of Mineral and Material Technology, Bhubaneswar, Odisha, India

Mishra P C - MITS School of Biotechnology, Bhubaneswar, Odisha, India

ABSTRACT
Leaves of Tagetes erecta andcannabis sativa traditionally practiced in the treatments of boils, dysentery, indigestion and various skin infections. The aim of this study was on the one hand to extract crude alkaloid from the leaves of Tagetes and Cannabis plants and on the other hand to evaluate the in vitro antibacterial activities of the crude extract against bacterial strains representative of skin, mouth and ear microflora and also against β-strain of E.coli bacteria responsible for urinary tract infection in human beings by disc diffusion method. The active ingredient of Tagetes erecta and Cannabis sativa was identified as alkaloid by Mayer’s and Dragendorff’s test. The antimicrobial activities were compared with two standard anbiotics Chloramphenicol and Tetracycline.However, this study revealed maximum growth inhibition and effectiveness was remarkably observed in the extracts of Cannabis sativa and then in Tagetes erecta. These results indicate that leaves have a potential broad spectrum antibacterial activity.

Keywords : Tagetes erecta, Cannabis sativa, Antibacterial activity, Disc diffusion, Active compound.

DOI: 10.6088/ijes.00202030045


© 2012 Copyright Das B and Mishra P C, licensee IPA.This is an open access article distributed under the Creative Commons Attribution License (2.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The electronic version of the article can be downloaded below.

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