International Journal of Environmental Sciences

Volume 2 Issue 3 2012           Pages:1742- 1753

Isolation and biological characterization of highly potent Tributyltin chloride resistant bacteria Alcaligenes sp. swo (Strain Sd) from marine water of Goa, India

Author Information:

Krishnamurthy.R - P.G.Studies & research in Microbiology,Brindavan college of science,Bangalore-560094.

Gopinath.S.M- Department of Biotechnology, Acharya Institute of Technology, Soladevanahalli, Bangalore

Dayananda.K.S - Department of Biotechnology, Acharya Institute of Technology, Soladevanahalli, Bangalore

Dubey S.K - Department of Microbiology, Goa University, Goa-403206

ABSTRACT
Water samples were collected from various sampling sites surrounding the ship building industries in Goa and plated on Zobell marine agar (ZMA), Nutrient agar (NA) and Mineral salt medium agar (MSMA) containing 0.1 mM tributyltin chloride (TBTC). The physico-chemical characteristics of the water samples were determined. The total viable counts of bacteria ranged from 58 × 102 cfu/ml in MSMA+TBTC to 40.6 × 106 cfu/ml in ZMA+TBTC. This clearly indicates predominance of TBTC tolerant and degrading bacterial isolates in marine water of Goa.  Although, several highly TBTC tolerant bacterial isolates were screened, one bacterial strain tolerated up to 7 mM TBTC and showed prominent growth in MSM supplemented upto 5mM TBTC. This clearly indicates that TBTC resistant bacterial isolate utilizes TBTC as a sole carbon source. Based on morphological, biochemical, and molecular characteristics (16S rDNA Sequence & taxonomic phenogram) this TBTC resistant isolate have been grouped in Alcaligenes sp. swo.

Keywords :Organotins, Alcaligenes sp. swo, Shipyard, Tributyltin, Carbon Source, 16 S ribosomal DNA.

DOI: 10.6088/ijes.00202030057


© 2012 Copyright Krishnamurthy.R et al, licensee IPA.This is an open access article distributed under the Creative Commons Attribution License (2.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The electronic version of the article can be downloaded below.

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