International Journal of Environmental Sciences

Volume 2 Issue 4 2012           Pages:2462- 2469

Aqueous two-phase poly (ethylene glycol) – Maltodextrin system for protein partitioning from shrimp waste: Influence of molecular weight and pH

Author Information:

Ramyadevi. D  Department of Chemical Engineering, National Institute of Technology, Tiruchirappalli - 620 015, India

Subathira. A  Department of Chemical Engineering, National Institute of Technology, Tiruchirappalli - 620 015, India

Saravanan S  Department of Chemical Engineering, National Institute of Technology, Tiruchirappalli - 620 015, India

ABSTRACT

The polysaccharide maltodextrin (Mdx) can provide a low cost alternative to substitute the fractioned dextran for the use with polyethylene glycol (PEG) in aqueous two-phase polymer - polymers systems. Aqueous two-phase system (ATPS) partitioning has been used to recover and concentrate proteins from Shrimp Waste and offers many advantages along with biomass removal. In this work we have studied the partitioning proteins in poly (ethylene glycol)(PEG)- maltodextrin (Mdx) aqueous two-phase systems. The influence of the polymers molecular weights, pH, and addition of sodium chloride were investigated. The protein partitioning decreases on increasing the molecular weight of PEG, whereas the partitioning increases with increase in pH and addition of sodium chloride of the system.

Keywords: Aqueous two-phase systems, Globular proteins, Poly (ethylene glycol), maltodextrin, Partition coefficient.

DOI: 10.6088/ijes.00202030127


© 2012 Copyright Ramyadevi. D et al, licensee IPA.This is an open access article distributed under the Creative Commons Attribution License (2.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The electronic version of the article can be downloaded below.

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