International Journal of Environmental Sciences

Volume 3 Issue 1 2012- July 2012        Pages: 36- 43               <<Previous    Next>>

Effective utilization of an aquatic weed Salvinia Molesta as a substarte for the production of Cellulase Enzyme –Eradication through utilization

Author Information:

Rahna. K. Rathnan, Sangeetha Gopal, Mariya Thomas, Seby Antony, Celine Thomas, Ambili Mechoor
Department of Biotechnology Engineering, Sahrdaya College of Engineering and Technology, Thrissur, Kerala

ABSTRACT

Most of the freshwater systems in our country are infested with one kind of aquatic weed or the other causing serious environmental problems. All efforts to control the growth and spread of these weeds have failed miserably and hence the concept of eradication through utilization is being adopted by many researchers. Cellulases are a group of hydrolytic enzymes capable of hydrolyzing the most abundant organic polymer i.e. cellulose to smaller sugar components including glucose subunits. The high cost of production of cellulase enzymes has hindered the industrial application of cellulose bioconversion. Production cost of Cellulases may be brought down by multifaceted approaches which include the use of cheap lignocellulosic substrates for fermentation production of the enzyme, the use of cost-efficient fermentation strategies and the optimization of the key factors of the fermentation process. This study is conducted to produce cellulase by wild type Pseudomonas strain, using Salvinia Molesta an aquatic weed as substrate. About twenty five bacteria were isolated from decayed Salvinia waste.  It was found that three isolates showed significant positive results with clear zone around the cultures. According to the morphological studies and staining techniques two isolates were found to be the genus of Bacillus and Pseudomonas. The highest crude enzyme production was observed at pH 7 and temperature of 50°C in a medium that was supplemented with Salvinia molesta as carbon source using Pseudomonas strain.

Keywords: Cellulase production, Salvinia molesta, wild type Pseudomonas stain, enzyme purification, enzyme characterization

DOI: 10.6088/ijes.2012030131005

© 2012 Copyright by the authors, licensee Integrated Publishing Association.This is an open access article distributed under the Creative Commons Attribution License (3.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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