International Journal of Environmental Sciences

Volume 3 Issue 1 2012- July 2012        Pages:98- 107               <<Previous    Next>>

Sub-acute toxicity effects of chlorpyrifos on acetylcholinesterase activity and recovery in the freshwater field crab Barytelphusa guerini

Author Information:

Madhusudan Reddy Narra, Rudra Reddy Regatte, Rajender Kodimyala
Department of Zoology, College of Science, Osmania University,
Hyderabad, Andhrapradesh, India

ABSTRACT

Chlorpyrifos (CPF), a common organophosphorus pesticide, which is widely used as an agrochemical for pest control. It causes a serious stress in fresh water fauna. It is known to inhibit acetylcholinesterase (AChE) in the fresh water field crab Barytelphusa guerini. In this context, we investigated the influence of sub-acute dose of CPF and the duration of exposure on AChE inhibition and subsequent recovery. The effect was estimated at 7, 14, 21 and 28 days of CPF intoxication on AChE activity in sensitive tissues i.e., gills, muscle, hepatopancreas and nervous tissue. CPF produced a significant long term inhibition of AChE activity in the crab. On 28th day of exposure the inhibition ranged between 54.2% and 71.2% in different tissues. The inhibition was significantly increased with increasing time and was found to be tissue specific. In the experimental period many behavioral changes such as loss of equilibrium, uncoordinated movement and increase in the levels of respiratory frequency were observed. In the second part, crabs were exposed to CPF for 28days and then allowed a period of recovery in the clean water. The recovery animal’s tissue samples were harvested at 3,6,9,12,15 and 18days and AChE activity was estimated. Crabs transferred to toxicant free water recovered the AChE activity in all the affected tissues up to 95 to 97% of control levels by the end of recovery period. 

Keywords: Chlorpyrifos, Organophosphorus, Crab, AChE, Recovery. 

DOI: 10.6088/ijes.2012030131011

© 2012 Copyright by the authors, licensee Integrated Publishing Association.This is an open access article distributed under the Creative Commons Attribution License (3.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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