International Journal of Environmental Sciences

Volume 3 Issue 3 2012- November 2012    Pages: 1116-1129  <<Previous    Next>>

Liquid formulations of Acetobacter diazotrophicus L1 and Herbaspirillum seropedicae J24 and their field trials on wheat

Author Information:

Patil Nita1, Gaikwad Pallavi2, Shinde Shubhangi3, Sonawane Hemlata4, Patil Neha5,  Kapadnis Balasaheb6

1- Department of Environmental Science, University of Pune, Ganeshkhind, Pune-411007, Maharashtra, India.

2, 3, 4, 5- Department of Microbiology, Annasaheb Magar Mahavidyalaya, Hadapsar, Pune-411028, Maharashtra, India.

6- Department of Microbiology, University of Pune, Ganeshkhind, Pune-411007, Maharashtra, India


Viability of the plant growth promoting bacteria A. diazotrophicus L1 and H. seropedicae J24 stored as liquid formulation was evaluated by studying the effect of growth medium, preservation medium and temperature. They were grown in molasses medium with the addition of NH4Cl (0.1% w/v). Cells were harvested at 10,000 rpm for 30 min and washed in phosphate buffer. They were then preserved at 4ºC and 25ºC with isotonic solutions of different substances. Efficacy of liquid formulations stored for different periods was tested on wheat crop by pot assay and field trials. The best growth medium was found to be 3% molasses supplemented with 0.1% (w/v) NH4Cl. Trehalose, gum arabica and polyethylene glycol 300 (PEG 300) provided better protective effects than other protective substances. Addition of L-ascorbic acid (0.02% w/v) to preservation media would improve the effectiveness of protective substances. After 8-9 months of storage at 4ºC, A. diazotrophicus L1 and H. seropedicae J24 cells preserved in gum arabica (5% w/v) and PEG 300 (5% w/v), respectively maintained their viability and efficacy of plant growth promotion. Bacterial cultures of different ages in these liquid formulations exhibited different plant growth promoting traits up to 240 days. Similarly, they significantly promoted the wheat plant growth compared to untreated control. There was significant difference in growth, grain yield and dry matter (straw yield) of wheat with these formulations. This ascertained the efficacy of A. diazotrophicus L1 and H. seropedicae J124 in liquid-based formulation. Thus, gum arabic and polyethylene glycol could be used to develop a stable liquid formulation of A. diazotrophicus L1 and H. seropedicae J24 as a commercial product.

Keywords: Liquid formulation, Gum Arabic, Polyethylene glycol, Bioinoculum, L-ascorbic acid, A. diazotrophicus, H. seropedicae


© 2012 Copyright by the authors, licensee Integrated Publishing Association.This is an open access article distributed under the Creative Commons Attribution License (3.0) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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